Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis.

Zha H; Li Y; Hu Z; Li J; Xie Y; Zhao M; Ren L; Zhang B
Synthetic and systems biotechnology 2026 · China · PubMed · DOI · PMC

Results (8 findings)

• SDH1 knockout + SDH3 knockout + SDH5 knockout + SDH4A knockout + SDH2 knockout → increases glycerol byproducts − Negative
  host K. marxianus · Zha H 2026 · details
• SDH1 knockout + SDH3 knockout + SDH5 knockout + SDH4A knockout + SDH2 knockout → increases acetate byproducts − Negative
  host K. marxianus · Zha H 2026 · details
• KU70 deletion → increases single-gene knockout efficiency + Positive
  host K. marxianus · Zha H 2026 · details
• SDH1 knockout + SDH3 knockout + SDH5 knockout + SDH4A knockout + SDH2 knockout → increases succinic acid production + Positive
  host K. marxianus · Zha H 2026 · details
• GPD1 knockout + ACH1 knockout + ADH2A knockout + NDE1 overexpression → increases succinic acid titer + Positive
  host K. marxianus · strain YZH43 · Zha H 2026 · 32.38 g/L · details
• GPD1 knockout + ACH1 knockout + ADH2A knockout + NDE1 overexpression → decreases glycerol production + Positive
  host K. marxianus · strain YZH43 · Zha H 2026 · 67.5 % · details
• GPD1 knockout + ACH1 knockout + ADH2A knockout + NDE1 overexpression → decreases ethanol production + Positive
  host K. marxianus · strain YZH43 · Zha H 2026 · 60.79 % · details
• GPD1 knockout + ACH1 knockout + ADH2A knockout + NDE1 overexpression → decreases acetate production + Positive
  host K. marxianus · strain YZH43 · Zha H 2026 · 89.24 % · details

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