INU1

Locus tag KLMA_10518 MARXBASE-G000045

27 findings (25 positive, 2 negative).

Also known as: INU, INU1A, SUC2

Named in the papers as: INU, INU1A

The literature name INU1 (inulinase) for the locus RefSeq annotates as SUC2, beta-fructofuranosidase (556 aa, chr 1). It is the reciprocal best hit of S. cerevisiae SUC2 (YIL162W; 52% identity, 95-99% mutual coverage), so the ortholog is assigned normally. The curator also reports an extra C-terminal inulin-binding domain, which the alignment does not show. INU and INU1A (literature names) are the same gene and are merged into INU1.

S. cerevisiae ortholog

Results

enzyme activity (2 positive)

• INU1 heterologous expression → increases inulinase activity + Positive
  host Aurantiochytrium sp. · strain Aurantiochytrium sp. recombinant T39 · Yu XJ 2020 · < 50.1 U/mL · details
• INU1 heterologous expression → increases extracellular inulase activity + Positive
  host Saccharomyces cerevisiae · Brevnova EE 1998 · details

gene expression (3 positive)

• INU1 point mutation → increases mRNA expression + Positive
  host K. marxianus · Zhou J 2018 · details
• INU1 truncation → increases promoter strength + Positive
  host Saccharomyces cerevisiae · strain S288C (ATCC 204508) · Gao J 2017 · details
• INU1 truncation → decreases promoter response to high glucose + Positive
  host Saccharomyces cerevisiae · strain S288C (ATCC 204508) · Gao J 2017 · details

growth (1 positive)

• INU1 heterologous expression → increases growth in inulin + Positive
  host Saccharomyces cerevisiae · Brevnova EE 1998 · details

product formation (7 positive)

• INU1 heterologous expression + GSH1 overexpression + GSH2 overexpression → increases glutathione titer + Positive
  host Yarrowia lipolytica · Do DTH 2020 · 644 nmol/mg protein · details
• INU1 heterologous expression + GSH1 overexpression + GSH2 overexpression → increases glutathione productivity + Positive
  host Yarrowia lipolytica · Do DTH 2020 · 510 µmol/gDCW · details
• INU1 heterologous expression → increases citric acid titer + Positive
  host Yarrowia lipolytica · strain Y. lipolytica AWG7 INU 8 · Rakicka M 2019 · > 200 g dm-3 · details
• INU1 heterologous expression → increases lipid content + Positive
  host Aureobasidium melanogenum · strain P10 · Li YF 2018 · 66.2 % · details
• INU1 heterologous expression + PGK1 promoter swap + MFΑ1 heterologous expression → increases ethanol titer from inulin + Positive
  host Saccharomyces cerevisiae · strain S. cerevisiae D452-2 · Hong SJ 2015 · 80.2 g/L · details
• INU1 heterologous expression → increases ethanol titer from inulin + Positive
  host Saccharomyces cerevisiae · strain S. cerevisiae D452-2/p426PM · Hong SJ 2015 · 80.2 g/L · details
• HXK1 knockout + HXK2 knockout + INU1 heterologous expression → increases fructose accumulation + Positive
  host Saccharomyces cerevisiae · Brevnova EE 1998 · details

protein production (11 positive, 2 negative)

• INU1 truncation → decreases alpha-galactosidase production level − Negative
  host K. marxianus · strain CBS 6556 · Bergkamp RJ 1993 · details
• INU1 truncation → decreases alpha-galactosidase secretion efficiency − Negative
  host K. marxianus · strain CBS 6556 · Bergkamp RJ 1993 · details
• INU1 knockout → increases scFv production + Positive
  host K. marxianus · Nambu-Nishida Y 2018 · details
• INU1 point mutation → increases Est1E secretory expression + Positive
  host K. marxianus · Zhou J 2018 · details
• INU1 point mutation → increases secretory expression of other lignocellulolytic enzymes + Positive
  host K. marxianus · Zhou J 2018 · details
• INU1 heterologous expression → increases inulinase production + Positive
  host Aureobasidium melanogenum · strain P10 · Li YF 2018 · 28.5 U/mL (baseline 8.62 U/mL, wild-type P10) · details
• INU1 heterologous expression → increases exo-inulinase production + Positive
  host Pichia pastoris · strain GS115 · Li Y 2015 · details
• GOX heterologous expression + INU1 promoter swap → increases secreted GOX expression + Positive
  host K. marxianus · strain CBS 6556 · Rocha SN 2010 · 1552 units per gram dry cell weight · details
• INU1 heterologous expression → increases inulinase production + Positive
  host Saccharomyces cerevisiae · Lim SH 2010 · 34.6 U/ml 13-fold · details
• LC1 heterologous expression + INU1 heterologous expression → increases LC1 secretion efficiency with INU signal peptides + Positive
  host Saccharomyces cerevisiae · Chung BH 1996 · ≈ 95 % (baseline 10 %, MFalpha1 leader peptide-containing vector) · details
• IL-2 heterologous expression + INU1 heterologous expression → increases IL-2 secretion with INU signal peptides + Positive
  host Saccharomyces cerevisiae · Chung BH 1996 · details
• INU1 heterologous expression → increases alpha 1-antitrypsin secretion + Positive
  host Saccharomyces cerevisiae · Kang HA 1996 · ≈ 75 mg l-1 · details
• INU1 heterologous expression → increases LC1 secretion efficiency + Positive
  host Saccharomyces cerevisiae · Chung BH 1996 · ≈ 95 % · details

substrate utilization (1 positive)

• INU1 heterologous expression → increases growth on inulin + Positive
  host Saccharomyces cerevisiae · Laloux O 1991 · details