Highly Multiplexed CRISPRi Repression of Respiratory Functions Enhances Mitochondrial Localized Ethyl Acetate Biosynthesis in Kluyveromyces marxianus.

Löbs AK; Schwartz C; Thorwall S; Wheeldon I
ACS synthetic biology 2018 · United States · PubMed · DOI

Results (5 findings)

• EAT1 knockout → decreases ethyl acetate production + Positive
  host K. marxianus · Löbs AK 2018 · details
• EAT1 knockout → decreases isoamyl acetate production + Positive
  host K. marxianus · Löbs AK 2018 · details
• EAT1 knockout → decreases phenylethyl acetate production + Positive
  host K. marxianus · Löbs AK 2018 · details
• EAT1 truncation → decreases ester biosynthesis + Positive
  host K. marxianus · Löbs AK 2018 · details
• ACO2B knockdown + SDH2 knockdown + RIP1 knockdown + MSS51 knockdown → increases ethyl acetate productivity + Positive
  host K. marxianus · Löbs AK 2018 · 3.8-fold · details

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