Reprogramming a Hgt-family Kluyveromyces marxianus sugar transporter by site-directed mutagenesis to enable co-consumption of glucose and xylose.

Donzella L; Belloch-Molina C; Morrissey JP; Sousa MJ
FEMS yeast research 2026 · Portugal · PubMed · DOI · PMC

Results (4 findings)

• KMAR_10 531 point mutation → decreases glucose affinity + Positive
  host K. marxianus · Donzella L 2026 · 20-fold · details
• KMAR_10 531 point mutation → increases xylose affinity + Positive
  host K. marxianus · Donzella L 2026 · 14.9 mM (baseline 46.9 mM, native transporter) · details
• KMAR_10 531 point mutation → increases glucose-xylose co-consumption + Positive
  host K. marxianus · Donzella L 2026 · 90 % · details
• KMAR_10 531 point mutation → increases biomass + Positive
  host K. marxianus · Donzella L 2026 · details

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