Relevance of aromatic and polar amino acids in the specificity of Inulinase ISO3 from Kluyveromyces marxianus: A molecular dynamics approach with an experimental verification.

Trapala J; González-Andrade M; Olvera C; Cayetano-Cruz M; Sanz-Aparicio J; Jimenez-Ortega E; Bustos-Jaimes I; Montiel C
International journal of biological macromolecules 2023 · Mexico · PubMed · DOI

Results (4 findings)

• INU-ISO3 point mutation → decreases inulinase activity on fructans (Trp79Ala) + Positive
  host Komagataella pastoris · Trapala J 2023 · details
• INU-ISO3 point mutation → decreases activity on sucrose (Trp79Ala) + Positive
  host Komagataella pastoris · Trapala J 2023 · 98 % · details
• INU-ISO3 point mutation → increases invertase activity (Phe113Ala) + Positive
  host Komagataella pastoris · Trapala J 2023 · details
• INU-ISO3 point mutation → increases invertase activity (Gln236Ala) + Positive
  host Komagataella pastoris · Trapala J 2023 · details

Cite this paper