Tuning enzymatic properties by protein engineering toward catalytic tetrad of carbonyl reductase.

Cheng F; Zhai QY; Gao XF; Liu HT; Qiu S; Wang YJ; Zheng YG
Biotechnology and bioengineering 2021 · China · PubMed · DOI

Results (3 findings)

• KMCR point mutation → increases catalytic efficiency (kcat/KM) + Positive
  host K. marxianus · Cheng F 2021 · 47.3 · details
• KMCR point mutation → increases stereoselectivity + Positive
  host K. marxianus · Cheng F 2021 · > 99.5 % (baseline 66.7 %, KmCR_WT) · details
• KMCR point mutation → increases substrate scope + Positive
  host K. marxianus · Cheng F 2021 · details

Cite this paper