MarxBaseK. marxianus metabolic engineering results

INU-ISO3 point mutation: inulinase activity on fructans (Trp79Ala)

Positive AI-extracted, curator-verified MARXBASE-R000598

GeneINU-ISO3 Enzyme or protein label, not a gene symbol
Gene fromK. marxianus
Changepoint mutation
Effectdecreases
Phenotypeinulinase activity on fructans (Trp79Ala) (enzyme activity)
Class Positive
HostKomagataella pastoris
Strainnot stated
ProductSecreted enzyme (Enzymes)
Condition tagfructans

Measured

Size as writtentotal loss of activity
Metricactivity

Evidence

Evidence sentences are not included in this release.

Paper

Relevance of aromatic and polar amino acids in the specificity of Inulinase ISO3 from Kluyveromyces marxianus: A molecular dynamics approach with an experimental verification.
Trapala J; González-Andrade M; Olvera C; Cayetano-Cruz M; Sanz-Aparicio J; Jimenez-Ortega E; Bustos-Jaimes I; Montiel C
International journal of biological macromolecules 2023 · PubMed · DOI

Cite as: MarxBase result MARXBASE-R000598, release 1.0.