← Enzymes
Secreted enzyme
Enzymes · 49 findings (34 positive, 9 negative, 6 context-dependent)
What worked
MNN11 deletion →
increases protein secretion
+ Positive PGU1 overexpression →
increases endoPG production
+ Positive YJR107W heterologous expression →
increases lipase specific activity
+ Positive PIR heterologous expression →
increases amylase production
+ Positive INU-ISO3 point mutation →
decreases inulinase activity on fructans (Trp79Ala)
+ Positive INU-ISO3 point mutation →
decreases activity on sucrose (Trp79Ala)
+ Positive INU-ISO3 point mutation →
increases invertase activity (Phe113Ala)
+ Positive INU-ISO3 point mutation →
increases invertase activity (Gln236Ala)
+ Positive PDC1 overexpression →
increases secretion of heterologous proteins
+ Positive PGK overexpression →
increases secretion of heterologous proteins
+ Positive VID28 overexpression →
increases secretion of heterologous proteins
+ Positive CAJ1 overexpression →
increases secretion of heterologous proteins
+ Positive MET13 overexpression →
increases secretion of heterologous proteins
+ Positive ESR1 deletion →
increases secretion of heterologous proteins
+ Positive HRD3 deletion →
increases secretion of heterologous proteins
+ Positive USA1 deletion →
increases secretion of heterologous proteins
+ Positive HRD3 deletion →
increases extracellular endoxylanase activity
+ Positive HRD3 deletion →
increases extracellular glucanase activity
+ Positive INU point mutation →
increases catalytic activity (S124Y)
+ Positive INU point mutation →
increases catalytic efficiency (S124Y)
+ Positive INU point mutation →
increases catalytic activity under acidic pH (N158S)
+ Positive INU point mutation →
increases substrate preference for inulin over sucrose (Q215V)
+ Positive AKR mutagenesis evolution →
increases enzyme activity toward (5R)-1
+ Positive INU1 heterologous expression →
increases inulinase production
+ Positive host Aureobasidium melanogenum · strain
P10 ·
Li YF 2018 · 28.5 U/mL (baseline 8.62 U/mL, wild-type P10) ·
detailsUOX heterologous expression →
increases tag-free uricase purification yield
+ Positive INU1 heterologous expression →
increases exo-inulinase production
+ Positive INU heterologous expression →
increases exo-inulinase expression
+ Positive INU heterologous expression →
increases extracellular inulinase activity
+ Positive GOX heterologous expression +
INU1 promoter swap →
increases secreted GOX expression
+ Positive INU1 heterologous expression →
increases inulinase production
+ Positive EPG1-2 heterologous expression →
increases endopolygalacturonase production
+ Positive ALPHA-AMYLASE heterologous expression →
increases alpha-amylase expression
+ Positive SOD1 overexpression →
increases SOD volumetric yield
+ Positive AGA1 heterologous expression →
increases alpha-galactosidase production
+ Positive
What failed or depended on conditions
MNN11 deletion →
increases alpha-galactosidase secretory activity
∼ Context-dependent MNN11 deletion →
increases endoxylanase secretory activity
∼ Context-dependent MNN11 deletion →
increases beta-glucanase secretory activity
∼ Context-dependent ESR1 overexpression →
decreases secretion of heterologous proteins
− Negative HRD3 overexpression →
decreases secretion of heterologous proteins
− Negative USA1 overexpression →
decreases secretion of heterologous proteins
− Negative YET3 overexpression →
decreases secretion of heterologous proteins
− Negative YET3 deletion →
no_effect secretion of heterologous proteins
− Negative GAA heterologous expression →
increases protein secretion
∼ Context-dependent SOD1 heterologous expression →
increases protein secretion
∼ Context-dependent SOD1 heterologous expression →
increases secreted SOD volumetric activity
∼ Context-dependent PGK promoter swap →
decreases alpha-galactosidase production
− Negative GAL7 promoter swap →
decreases alpha-galactosidase production
− Negative INU1 truncation →
decreases alpha-galactosidase production level
− Negative INU1 truncation →
decreases alpha-galactosidase secretion efficiency
− Negative