Expression of an alpha-galactosidase gene under control of the homologous inulinase promoter in Kluyveromyces marxianus.
Bergkamp RJ; Bootsman TC; Toschka HY; Mooren AT; Kox L; Verbakel JM; Geerse RH; Planta RJ
Applied microbiology and biotechnology 1993 · Netherlands · PubMed · DOI
Results (5 findings)
PGK promoter swap →
decreases alpha-galactosidase production
− Negative GAL7 promoter swap →
decreases alpha-galactosidase production
− Negative INU1 truncation →
decreases alpha-galactosidase production level
− Negative INU1 truncation →
decreases alpha-galactosidase secretion efficiency
− Negative AGA1 heterologous expression →
increases alpha-galactosidase production
+ Positive
Cite this paper