Multistep engineering of the secretory pathway for enhanced leghemoglobin expression in Kluyveromyces marxianus.

Wu X; Feng D; Chen H; Yin A; Tian T; Zhou J; Yu Y; Lu H
Bioresource technology 2026 · China · PubMed · DOI

Results (10 findings)

• MNS1 knockout → increases LBA secretion + Positive
  host K. marxianus · Wu X 2026 · details
• MNL1 knockout → increases LBA secretion + Positive
  host K. marxianus · Wu X 2026 · details
• SEC23 overexpression → increases LBA secretion + Positive
  host K. marxianus · Wu X 2026 · details
• SAR1 overexpression → increases LBA secretion + Positive
  host K. marxianus · Wu X 2026 · details
• ERV29 overexpression → increases LBA secretion + Positive
  host K. marxianus · Wu X 2026 · details
• EMP47 overexpression → increases LBA secretion + Positive
  host K. marxianus · Wu X 2026 · details
• BET1 overexpression → increases LBA secretion + Positive
  host K. marxianus · Wu X 2026 · details
• PRB1 deletion → increases LBA secretion + Positive
  host K. marxianus · Wu X 2026 · details
• HMX1 knockdown + MNS1 deletion + PRB1 deletion + SEC23 overexpression + SAR1 overexpression → increases LBA secretion titer + Positive
  host K. marxianus · Wu X 2026 · 1.02 g/L 2.3-fold · details
• OST1 other → increases LBA secretion + Positive
  host K. marxianus · Wu X 2026 · details

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