Tri-Functional CRISPR Screen Reveals Overexpression of QDR2 and QDR3 Transporters Increase Fumaric Acid Production in Kluyveromyces marxianus.

Thornbury M; Omran RP; Kumar L; Knoops A; Abushahin R; Whiteway M; Martin VJJ
ACS synthetic biology 2025 · Canada · PubMed · DOI

Results (4 findings)

• ATP7 deletion → increases fumaric acid tolerance + Positive
  host K. marxianus · strain Y-1190 · Thornbury M 2025 · details
• QDR2 overexpression → increases fumaric acid tolerance + Positive
  host K. marxianus · strain Y-1190 · Thornbury M 2025 · details
• QDR3 overexpression → increases fumaric acid tolerance + Positive
  host K. marxianus · strain Y-1190 · Thornbury M 2025 · details
• QDR2 overexpression + QDR3 overexpression → increases fumaric acid titer + Positive
  host K. marxianus · Thornbury M 2025 · 2.16 g L-1 (baseline 0.26 g L-1) · details

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