Engineering better catalytic activity and acidic adaptation into Kluyveromyces marxianus exoinulinase using site-directed mutagenesis.

Wang CH; Xiong WP; Huang C; Li XM; Wang QY; Huang RB
Journal of the science of food and agriculture 2021 · China · PubMed · DOI

Results (4 findings)

• INU point mutation → increases catalytic activity (S124Y) + Positive
  host Pichia pastoris · strain GS115 · Wang CH 2021 · 688.4 59% change · details
• INU point mutation → increases catalytic efficiency (S124Y) + Positive
  host Pichia pastoris · strain GS115 · Wang CH 2021 · 568.93 99% change · details
• INU point mutation → increases catalytic activity under acidic pH (N158S) + Positive
  host Pichia pastoris · strain GS115 · Wang CH 2021 · 464.06 · details
• INU point mutation → increases substrate preference for inulin over sucrose (Q215V) + Positive
  host Pichia pastoris · strain GS115 · Wang CH 2021 · 5.56-fold · details

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