Efficient purification of a recombinant tag-free thermostable Kluyveromyces marxianus uricase by pH-induced self-cleavage of intein and expression in Escherichia coli.

Wang B; Luo L; Wang D; Ding R; Hong J
3 Biotech 2018 · China · PubMed · DOI · PMC

Results (1 findings)

• UOX heterologous expression → increases tag-free uricase purification yield + Positive
  host Escherichia coli · strain BL21 (DE3) · Wang B 2018 · 77.11 % · details

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