Rational design of Kluyveromyces marxianus ZJB14056 aldo-keto reductase KmAKR to enhance diastereoselectivity and activity.

Wang YJ; Ying BB; Shen W; Zheng RC; Zheng YG
Enzyme and microbial technology 2017 · China · PubMed · DOI

Results (3 findings)

• AKR point mutation → increases diastereoselectivity toward (5R)-1a + Positive
  host K. marxianus · Wang YJ 2017 · 80.5 % · details
• AKR point mutation → increases kcat toward (5R)-1a + Positive
  host K. marxianus · Wang YJ 2017 · 10.36 2.8-fold · details
• AKR point mutation → increases kcat/Km toward (5R)-1a + Positive
  host K. marxianus · Wang YJ 2017 · 6.56 3.9-fold · details

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