LAC4

Locus tag KLMA_30009 MARXBASE-G000041

10 findings (9 positive, 1 negative).

Results

enzyme activity (1 positive)

• LAC4 knockout → decreases beta-galactosidase activity + Positive
  host K. marxianus · strain CBS 6556 · Ribeiro O 2007 · details

gene expression (2 positive)

• LAC4 point mutation → decreases LAC4 expression leakage in glucose + Positive
  host K. marxianus · Liu B 2022 · details
• LAC4 point mutation → no_effect LAC4 induction by lactose + Positive
  host K. marxianus · Liu B 2022 · details

genetic tools (2 positive)

• LAC4 other → increases recombination frequency between loxP sites + Positive
  host K. marxianus · Ren H 2022 · 99 % · details
• LAC4 other → increases recombination frequency between loxPsym sites + Positive
  host K. marxianus · Ren H 2022 · 73 % · details

growth (1 negative)

• LAC4 heterologous expression + LAC12 heterologous expression + NTH1 deletion + ATH1 deletion → decreases growth − Negative
  host Saccharomyces cerevisiae · strain AY-51024A · Zou J 2013 · details

product formation (2 positive)

• LAC4 heterologous expression + LAC12 heterologous expression + MIG1 deletion + NTH1 deletion → increases ethanol production from lactose in cheese whey + Positive
  host Saccharomyces cerevisiae · strain AY-5 · Zou J 2013 · 63.3 g l(-1) · details
• LAC4 heterologous expression + LAC12 heterologous expression → increases ethanol productivity from lactose + Positive
  host Saccharomyces cerevisiae · Domingues L 1999 · details

substrate utilization (2 positive)

• LAC4 overexpression + LAC12 overexpression → increases lactose uptake and conversion + Positive
  host Saccharomyces cerevisiae · Zou J 2021 · details
• LAC4 heterologous expression + LAC12 heterologous expression → increases lactose metabolization + Positive
  host Saccharomyces cerevisiae · Domingues L 2001 · 50 g/L · details